The Preserve contains some of the most biologically diverse habitats in this region of Alabama. The waters of Turkey Creek are home to three endangered species of fish: the Vermilion Darter (Etheostoma chermocki), the Watercress Darter (Etheostoma nuchale), and the Rush Darter (Etheostoma phytophilum). The Vermilion Darter occurs only in Turkey Creek and nowhere else in the world.

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